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. 2015 Aug 3;16(1):146. doi: 10.1186/s13059-015-0718-x

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© Burman et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Automated nucleus segmentation and FISH signal detection. a, c, e, g, i Maximal projections of 40× confocal image z-stacks of Mac2A cells stained with the hiBA-ALK probe set. The overlay represents a composite image of the Green (Alexa488), Red (Alexa568) and FarRed (Cy5) channels. The inset in each panel represents a magnified image of representative Mac2A cells. DAPI 4',6-diamidino-2-phenylindole. Scale bars 10 μm. b, d, f, h, j In silico generated images representing the detected nucleus ROI (yellow) and the FISH signal ROIs (in green, red and blue)