Fig. 9.

Exacerbated Th2 and reduced effector CD8⁺ T cells responses following reinfection with rA2-19F. Six days post reinfection, lung cells were isolated and stained with CD3, CD4, CD8, IFNγ, and IL4 for flow cytometry; and BALF were isolated and cytokines were measured. a CD4⁺ T cells expressing IFNγ or IL4. N = 5-7. b CD4⁺ T cell subsets (Th1: IFNγ + IL4-; Th2: IFNγ-IL4+; multi-functional Th: IFNγ + IL4+). c Representative flow plot of (a). d CD8⁺ T cell expressing IFNγ or IL4. N = 5-7. e Representative flow plot of (c). f IFNγ in the BALF. N = 5-8. g IL12(p40) in the BALF. N = 6-8. h IL4 in the BALF. N = 5-8. i IL13 in the BALF. N = 23–24. Sham/Sham: mice initially infected with media as neonates and reinfected with media as young adults; A2/A2: mice initially infected with A2 as neonates and reinfected with A2 as young adults; rA2-19F/rA2-19F: mice initially infected with rA2-19F as neonates and reinfected with rA2-19F as young adults. These figures are representative of 2 independent experiments. *:p < 0.05