Fig. 1.

Acetylation of histone lysine residues in asthmatic and healthy airways. Airway sections from asthmatic and healthy patients were formalin fixed, embedded in paraffin and then sectioned for immunohistochemical analysis. Sections were stained for acetylation of H3K14ac (a, b), H3K18ac (c, d), H3K27ac (e, f), H4K8ac (g, h), H4K12ac (i, j) or H4K16ac (k, l). Scale bar is equal to 50 μm. The expression of and the % of positive nuclei stained for H3K18ac within the epithelium was quantified for both healthy (H, white bar) and asthmatic (A, blue bar) subjects (m, n). Data are expressed as % of positive area of airway epithelium ± SEM (n = 6) and % positive nuclei ± SEM (n = 6). A two-tailed unpaired t-test was performed, *indicates p < 0.05