Fig. 3.

Association of H3K18ac and H3K4me2 at the ΔNp63 locus. Chromatin immunoprecipitation for H3K18ac, H3K4me2, and IgG was performed on AECs from asthmatic and healthy subjects. Primers located around the transcription start site (TSS) of ΔNp63 (a) were used for real time PCR targeting the ΔNp63-I (b) and the ΔNp63-II (c) loci. The presence of each histone modification and negative control was quantified and expressed as % Input at the target locus ± SEM (n = 5). A two-tailed unpaired t-test was performed to determine differences in % Input between asthmatic and healthy AECs for each locus, * indicates p < 0.05