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A representative Western blot of total tissue protein (100 μg) extracted from inner medulla (IM), outer medulla (OM) and cortex (CT) of the kidney of SHR (H) and WKY rats (C). Rat pituitary (bNOS), induced macrophage (iNOS) and human endothelial cell (ecNOS) were added as positive control (+) for each NOS isoform.
