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. 2015 Aug 11;6:623. doi: 10.3389/fpls.2015.00623

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Copyright © 2015 Ali, Magne, Chen, Obradovic, Jamshaid, Wang, Bélair and Moffett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GrSPRYSEC-15 induces a hypersensitive response in N. tabacum and chlorosis in N. benthamiana. (A) N. tabacum leaves were infiltrated with Agrobacterium carrying pEAQ35S expressing AtRx, INF1, GrSPRYSEC-15, or GFP as indicated. Leaves were photographed at 4 DPI. (B) N. tabacum inoculated by agroinfiltration with PVX-GFP vector or (C) PVX-GrSPRYSEC-15. Pictures were taken at 14 DPI. Insets show close ups of leaves displaying typical PVX symptoms and the sites of agroinfiltration of PVX-GrSPRYSEC-15 are seen as yellowish spots in (C). (D) N. benthamiana inoculated with PVX-GFP vector or (E) PVX-GrSPRYSEC-15 or (F) PVX-GrSPRYSEC-5. Plants were photographed at 21 DPI.