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. 2015 Aug 11;6:623. doi: 10.3389/fpls.2015.00623

Figure 2.

Figure 2

Suppression of cell death induced by NB-LRR and PiNPP proteins by GrSPRYSECs in N. benthamiana and N. tabacum. (A) N. benthamiana leaves were co-infiltrated with Agrobacterium carrying binary vectors expressing Rx, CP and P38 together with either empty vector (EV, left hand side) or the indicated effectors expressed from pEAQ35S (right hand side). Three days DPI, leaves were decolorized with methanol to highlight cell death reactions. (B) N. tabacum leaves were co-infiltrated with Agrobacterium containing binary vectors expressing AtRx and P38 together with either empty vector (EV, left hand side) or the indicated effectors expressed from pEAQ35S (right hand side). (C,D) N. tabacum leaves were co-infiltrated with Agrobacterium carrying expression vectors for PiNPP and P38 together with either empty vector (EV, left hand side) or the indicated effectors (right hand side). Effectors were expressed from a PVX expression vector in (C,D). Cell death symptoms were scored at 3-5 DPI and the pictures were taken at 5 DPI.