Skip to main content
. 2015 Aug 11;6:623. doi: 10.3389/fpls.2015.00623

Figure 3.

Figure 3

GrSPRYSECs suppress Rx-mediated resistance to PVX. N. benthamiana leaves were co-infiltrated with Agrobacterium carrying binary vectors expressing PVX-GFP and Rx together with (A) 1, GrSPRYSEC-5; 2, empty vector; 3, GrSPRYSEC-8; 4, empty vector; 5 GrSPRYSEC-15; 6, empty vector; 7, GrSPRYSEC-19; 8, empty vector; and Rx replaced with empty vector. (B) 1, GrSPRYSEC-18; 2, empty vector; 3, GrSPRYSEC-4; 4, empty vector; GFP expression was visualized and photographed under UV illumination at 4 DPI. (C,D) Anti GFP immune blotting was performed on total protein samples taken at 4 DPI from infiltrated N. benthamiana leaf patches expressing the different construct combinations as described in (A,B). Numbering corresponds to the number on the leaf above each blot. Ponceau staining (lower panel) was used to show equal loading.