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. 2015 Aug 11;6:623. doi: 10.3389/fpls.2015.00623

Figure 4.

Figure 4

GrSPRYSECs proteins suppress virus resistance mediated by the N gene. N. benthamiana leaves were co-infiltrated with Agrobacterium carrying binary vectors expressing PVX-GFP, N, and P50 together with (A) 1, GrSPRYSEC-5; 2, empty vector; 3, GrSPRYSEC-8; 4, empty vector; 5, GrSPRYSEC-15; 6, empty vector; 7, P0; 8, P38. (B) 1, GrSPRYSEC-19; 2, empty vector; 3, GrSPRYSEC-4; 4, empty vector; 5, P0; 6, P38. GFP expression was visualized under UV illumination at 4 DPI. (C,D) Anti GFP immune blotting was performed on total protein samples taken at 4 DPI from N. benthamiana leaf patches co-expressing the combinations of constructs described in (A,B). The number on the blot corresponds to the number on the leaf above each blot. Ponceau staining (lower panel) was used to show equal loading.