Figure 3.

The effect of 1,25(OH)₂D₃, 25(OH)D₃ and calcipotriol on the proliferation of CRC cell lines. The effects of (A–F) 1,25(OH)₂D₃, (G–L) 25(OH)D₃ and (M–R) calcipotriol on proliferation of LoVo, HCT116 and HT29 cells were measured by MTT and SRB assays. Cells were incubated in medium (see Materials and methods) with varying concentrations (10⁻⁶ to 10⁻¹¹ M) of the vitamin D analogue for 48 h prior to the SRB and MTT assays. Significance compared to the vehicle-treated control: ^*P<0.05, ^**P<0.01, ^***P<0.001, ^****P<0.0001; mean ± SD.