Table I.
The PCR primers.
| GENE | Protein name | Primer sequence (5′-3′) | Amplicon size (nt) |
|---|---|---|---|
| ACTB | β-actin | GCTCGTCGTCGACAACGGCTC | |
| CAAACATGATCTGGGTCATCTTCT | 353 | ||
| VDR | Vitamin D receptor | CCAGTTCGTGTGAATGATGG | |
| GTCGTCCATGGTGAAGGA | 384 | ||
| PDIA3 | Protein disulfideisomerase-associated 3 | CTCCGACGTGCTAGAACTCA | |
| CAGGTGTTAGTGTTGGCAGT | 204 | ||
| CYP24A1 | 24-hydroxylase | GCAGCCTAGTGCAGATTT | |
| ATTCACCCAGAACTGTTG | 335 | ||
| CYP24SV | 24-hydroxylase | TCCTGAAGTTGCAGCTGGAGT | |
| GAGCTCATCTATTCTGCCCATA | 215 | ||
| CYP2R1 | 25-hydroxylase | AGAGACCCAGAAGTGTTCCAT | |
| GTCTTTCAGCACAGATGAGGTA | 259 | ||
| CYP27B1 | 1α-hydroxylase | TGTTTGCATTTGCTCAGA | |
| CCGGGAGAGCTCATACAG | 227 |