Skip to main content
. 2015 Jul 9;47(3):891–899. doi: 10.3892/ijo.2015.3077

Figure 2.

Figure 2

Effects of survivin siRNA on apoptotic activity and cell viability in human MFH/UPS cell lines. (A) Survivin mRNA expression was evaluated by qPCR in siRNA transfected MFH/UPS cell lines after 72 h of siRNA transfection. Data represent mean ± SE of at least three independent experiments (*p<0.05). (B) Immunoblot analysis for survivin in siRNA-transfected MFH/UPS cell lines. (C) Immunoblot analysis for apoptosis-related proteins in siRNA-transfected Nara-H MFH/UPS cells. (D) DNA fragmentation was assessed by FACS analysis in siRNA-transfected Nara-H MFH/UPS cells. (E) Relative cell viability was assessed by WST-8 assay in siRNA-transfected MFH/UPS cell lines after 24, 48, 72, and 96 h of siRNA transfection. Data represent mean ± SE of at least three independent experiments (*p<0.05).