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. 2015 Jul 2;47(3):857–866. doi: 10.3892/ijo.2015.3069

Figure 6.

Figure 6

Expression and stimulation of TLR7 and TLR8 causes increased proliferation in TLR7+ and TLR8+ PANC1 cells. (A) Increased tumor size in subcutaneously injected Balb/c nude mice triggered by TLR7+ (n=5) and TLR8+ (n=5) PANC1 cells compared to empty vector PANC1 cells (n=4). (B) Significant increase in tumor volume caused by TLR7+ PANC1 cells in Balb/c nude mice compared to empty vector cells (**P<0.005). (C) Significantly accelerated proliferation of TLR7+ and TLR8+ PANC1 cells without (**P<0.002 and **P<0.005) and with R848 stimulation (***P<0.02 and *P<0.0001) compared to empty vector PANC1 cells analyzed by MTS assay. (D) Increased gene expression of Ki-67 in R848 stimulated TLR7+ and TLR8+ PANC1 (*P<0.0001 and **P<0.0005) cells compared to empty vector PANC1 cells. Data of three independent experiments are shown with standard deviation. Untreated PANC1 cells were standardized to baseline. The relative gene expression is expressed as 2−ΔΔCq.