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. Author manuscript; available in PMC: 2015 Aug 11.
Published in final edited form as: Anal Chem. 2013 Sep 26;85(20):9868–9876. doi: 10.1021/ac4028559

Figure 1. Reaction Scheme.

Figure 1

Our proposed reaction scheme describes how hybridization changes both DNA structure and silver cluster spectra. (Left) The composite strand has the 5’ cluster domain (S1 = CCCCAACTCCTT (black segment)) and one of five recognition sites (S212a = CCCGCCGCTGGA (red segment)). This single-stranded oligonucleotide hosts an ~11 silver atom with λmax = 400 nm (solid black line – left axis) and low emission (dashed red line – right axis). The cluster condenses its host strand. (Right) This cluster-laden strand hybridizes with its complementary strand (S2C12a = TCCAGCGGCGGG (green segment)). Cluster size is conserved but λmax shifts to 490 nm (solid black line – left axis) and strong green emission develops (dashed red line – right axis with 10X scale difference).