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. Author manuscript; available in PMC: 2015 Dec 25.
Published in final edited form as: Nature. 2015 Jun 17;522(7557):439–443. doi: 10.1038/nature14561

Fig. 4. EPF2, but not Stomagen, triggers downstream MAPK activation in Arabidopsis seedlings.

Fig. 4

(a, b) Differential MAPK activation in Arabidopsis wild-type seedlings treated with buffer only (a, Mock), MEPF2 (a, b), Stomagen (a), and heat-denatured MEPF2 (b) for respective time intervals (min). The blots were probed with anti-phosphoERK antibody (αERK) to detect phosphorylated MPK6 (pMPK6) and pMPK3 upon peptide treatment. *non-specific band. CBB, total proteins stained. Four and two biological replicates were performed for (a) and (b), respectively. (c) Confocal microscopy of Arabidopsis wild-type cotyledon abaxial epidermis treated with heat-denatured MEPF2 (top) and control, non-denatured MEPF2 (bottom). Scale bar, 40 μm. n=3 for each treatment.