| pKUT608 |
STOMAGEN in pENTR |
STOMAGEN cDNA |
pENTR |
KAN |
NA |
| pKMP127 |
proEst∷STOMAGEN in pER8 |
STOMAGEN cDNA |
pER8 |
SPEC/STREP |
HYG |
| pJSL92 |
ERL2 genomic ΔKinase in pENTR |
ERL2 genomic ΔKinase |
pENTR |
KAN |
NA |
| pJSL93 |
35S∷gERL2-ΔKinase-GFP in pGWB5 |
ERL2 genomic ΔKinase |
pGWB5 |
KAN/HYG |
KAN/HYG |
| pJSL73 |
FLS2ΔK in pENTR |
FLS2ΔK cDNA no stop |
pENTR |
KAN |
NA |
| pJSL75 |
35S:FLS2ΔK-GFP in pGWB5 |
FLS2ΔK cDNA no stop |
pGWB5 |
KAN/HYG |
KAN/HYG |
|
| Primer names |
Sequences (5′ to 3′) |
Purpose |
|
|
|
|
| EPFL9 1 XhoIf |
CACCTCGAGATGAAGCATGAA |
molecular cloning (pKUT608) |
|
|
|
| EPFL9 289 SpeI rc |
ACTAGTTATCTATGACAAACAC |
molecular cloning (pKUT608) |
|
|
|
| FLS2 1 (GW) F |
CACCATGAAGTTACTCTCAAAGACCTTTTTG |
molecular cloning (pJSL73) |
|
|
|
| FLS2 2625 rc |
GATGTTGGCACTGTTGAATGAATCTGTTGC |
molecular cloning (pJSL73) |
|
|
|
| FLS2 591 F |
TGTAGCAGCTGGTAACCAT |
Sequencing |
|
|
|
| eIF4A F |
AGCCAGTGAGAATCTTGGTGAAGC |
RT-PCR |
|
|
|
| eIF4A R |
CTAGTACGGCAGAGCAAACACAGC |
RT-PCR |
|
|
|
| STOMAGEN F |
TGTAGTTCAAGCCTCAAGACCTC |
RT-PCR |
|
|
|
| STOMAGEN R |
ACTCGTTGTACGTACAAGTTGGT |
RT-PCR |
|
|
|
| pER8 Term R |
TCGAAACCGATGATACGGACG |
RT-PCR |
|
|
|
| EPF1+207F |
ATGCCGTCTTGTGATGGTTAG |
RT-PCR |
|
|
|
| EPF1+315rc |
TCAAGGGACAGGGTAGGACTT |
RT-PCR |
|
|
|
| EPF2.1.cDNA.xhoI |
CACCCTCGAGATGACGAAGTTTGTACGCAAGT |
RT-PCR |
|
|
|
| EPF2.360.cDNA.ecoRI.rc2 |
CGGAATTCTAGCTCTAGATGGCACGTGATAG |
RT-PCR |
|
|
|
