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. Author manuscript; available in PMC: 2015 Dec 23.
Published in final edited form as: Nat Commun. 2015 Jun 23;6:7281. doi: 10.1038/ncomms8281

Figure 5. ΔCTL requires the membrane-anchoring CTC to invoke bulging and lysis.

Figure 5

(a) Schematics and representative phase contrast images of WT cells (FtsZ) and cells producing the indicated FtsZ variants and depleted of WT FtsZ for 5 h. ΔCTLC - FtsZ lacking the CTL and FtsA-binding CTC. MTS - membrane targeting sequence from E. coli MinD. ΔCTC - FtsZ lacking the CTC. Bar = 2 μm. (b) Immunoblots of lysates from strains in (a) grown with vanillate and glucose or with xylose for 5 h probed with FtsZ (top) or SpmX antisera. Positions of molecular weight markers (kDa) are indicated. (c) Growth and lysis of strains from (a). Cells were grown with xylose from time 0 and OD was monitored at 600 nm. Final plots were normalized for each data set and represent average data from three technical replicates. (d) ZapA-Venus fluorescence (yellow) overlaid on phase contrast (blue) images of cells producing the indicated FtsZ CTL variants and ZapA-Venus. Each variant was induced with xylose while depleting WT FtsZ for 5 h prior to imaging. Bar = 2 μm.