Skip to main content
. 2015 Aug 11;10(8):e0130198. doi: 10.1371/journal.pone.0130198

Fig 2. Pro-inflammatory phenotype in mast cells exposed to hyper-insulinemic conditions in vivo.

Fig 2

A, B. Suppressed beta-hexosaminidase release (A) and elevated LTC4 (B) release in BMMC from C57/BL6 mice maintained on normal or high fat diets (HFD). BMMC were generated from femurs and cultured in IL-3 and sub-optimal (0.5 ng/ml) insulin for 6 d after confirmation of Fc-RIalpha chain staining (not shown). FcεRI stimulation achieved by cross-linking of IgE-bound FcεRI with 200ng/ml KLH-DNP.