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. 2015 Aug 11;10(8):e0135239. doi: 10.1371/journal.pone.0135239

Fig 1. Identification and annotation of full length exo-1,3-β-glucanase gene (exo1) from P. insidiosum:

Fig 1

(A) known 924-bp partial sequence of the exo1 gene (accession number: GU994093); (B) Adaptor and (C) RACE PCR used to identify upstream and downstream regions of the partial exo1 gene from genomic DNA (gDNA) and complementary DNA (cDNA), respectively; (D) and (E) Full length exo1 coding sequence amplified from gDNA and cDNA, respectively [forward and reverse primers for PCR and sequencing (Table 1) are depicted above and below the sequence structure, respectively]; (F) Promoter [including the core-promoter sequence and the transcriptional start site (1+)], 5’-untranslated, open reading frame, and 3’-untranslated regions of the exo1 gene; (G) Exo1 protein structure showing signal peptide (SP), BglC domain, X8 domain, transmembrane region (TM), and positions and sequences of Peptide-A, -B, and -C. Numbers indicate amino acid position.