Fig 1. Time course of the effect of NECA (1μM) on the expression of mRNA of RGS2 (A), RGS3 (B) and RGS4 (C) in human astrocytoma cells U373 MG.

Cells were cultured, incubated with NECA and assayed for mRNA expression by quantitative realtime PCR as described in Materials and Methods. Data given are means of five replica culture dishes ±SEM. A repetition of the experiment gave a similar result. ** p<0.01, *** p<0.001, student’s t-test, two-tailed.