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. 2015 Aug 11;10(8):e0134934. doi: 10.1371/journal.pone.0134934

Fig 2. Effects of adenosine agonists and the adenosine A2B receptor antagonist MRS1706 (500 nM) on the expression of mRNA of RGS2 (A,B), RGS3 (C,D) and RGS4 (E,F) in human astrocytoma cells U373 MG.

Fig 2

Cells were cultured, incubated with agonists/antagonists and assayed for mRNA expression by quantitative realtime PCR as described in Materials and Methods. Data given are means of 5 experiments (NECA, NECA/MRS1706) or 2 experiments (CPA, IB-MECA, CGS21680) each done in five replica culture dishes.* p<0.05, ** p<0.01, *** p<0.001, student’s t-test, two-tailed.