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. 2015 Aug 11;10(8):e0134934. doi: 10.1371/journal.pone.0134934

Fig 3. Time course of the effect of NECA (1μM) on the expression of mRNA of RGS2 (A), RGS3 (B) and RGS4 (C) in rat astrocytes.

Fig 3

Cells were cultured, incubated with NECA and assayed for mRNA expression by quantitative realtime PCR as described in Materials and Methods. Data given in A and B are means of five replica culture dishes ±SEM. Two repetitions of the experiment gave similar results. Data given in C are means of 3 different experiments done in four replica culture dishes. *p<0.05, ** p<0.01, *** p<0.001, student’s t-test, two-tailed.