Fig 4. Effects of adenosine agonists and the adenosine A2A and A2B receptor antagonists, MRS1706 (500nM) and ZM241385 (1μM), on the expression of mRNA of RGS2 (A,B,C), RGS3 (D,E,F) and RGS4 (G) in rat astrocytes.

Cells were cultured, incubated with agonists/antagonists and assayed for mRNA expression by quantitative realtime PCR as described in Materials and Methods. Data given are means of 4 (NECA, CGS21680), 3 (CPA, IB-MECA, CGS21680/ZM241385) or 5 (NECA/MRS1706) experiments each done with 4 replica culture dishes. * p<0.05, ** p<0.01, *** p<0.001, student’s t-test, two-tailed.