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. 2015 Aug 11;10(8):e0134666. doi: 10.1371/journal.pone.0134666

Fig 5. SPC plays an important regulatory role in the expression of class I/III PI3K and the interaction among Bcl2, Beclin1 and Vps34.

Fig 5

(A) LV tissues were collected from rats at the end of 2h reperfusion. The immunoblotting for phosphor-Akt (Ser473), Akt, phosphor- mTOR (Ser2448) and mTOR were performed. n = 4 /group. (B) Immunoblotting was used to examine the expression levels of Vps34, Bcl2 and Beclin1. Representative protein images and quantitative analysis were shown. The blots for Tubulin were served as loading controls. n = 4 /group. (C) Effect of SPC on I/R-induced association between beclin1 and Vps34. Upper panel, Cell lysates from LV tissues were immunoprecipitated (IP) with Beclin1 antibody and immunoblotted (IB) for Vps34 and Beclin1; Lower panel, Lysates were immunoprecipitated with Vps34 antibody and immunoblotted for Vps34 and Beclin1. The columns and errors bars represent means ± SD. * P < 0.05 vs. SHAM group; # P < 0.05 vs. I/R group.