Table 1. Primers used for real-time RT-PCR.
| Gene | Primer sequences (5'-3') | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
| Bcl-xL | F: CTTACCTGAATGACCACCTAGAGC | 60 | 182 |
| R: CCGACTGAAGAGCGAACCC | |||
| Bax | F: CGGGACACGGAGGAGGTTT | 60 | 189 |
| R: CGAGTCGTATCGTCGGTTG | |||
| Casp3 | F: ACTGTGGGATTGAGACGG | 55 | 110 |
| R: GGAATAGTAACGAGGTGCTG | |||
| Dnmt1 | F: GGCAGACCACCATCACATC | 55 | 165 |
| R: GGAGCAGTCCGGCAACT | |||
| Dnmt3a | F: GGACAAGAATGCCACCAAATCA | 60 | 196 |
| R: CTTGCCGTCTCCGAACCA | |||
| Dnmt3b | F: GGGTGGAAAGACACGGGAT | 60 | 243 |
| R: TAGGAGCGTAGAAGCAAGGAA | |||
| Pou5f1 | F:GCTCACTTTGGGGGTTCTCT | 60 | 228 |
| R:TTGCCTCTACTCGGTTCTC | |||
| Cdx2 | F: GCAAAGGAAAGGAAAATCAACAA | 60 | 120 |
| R: GGGCTCTGGGACGCTTCT | |||
| Rpl19 | F: GCTTGCCTCCAGTGTCCTC | 60 | 179 |
| R: GGCGTTGGCGATTTCAT |