Figure 6.

Effect of maternal gestational diabetes mellitus (GDM) and postnatal diet on gene expression in the livers of the male offspring

A and B, acetyl-coenzyme A carboxylase-1 (Acca) and Acc-2 (Accb) mRNA expression. C and D, sterol response element binding protein (Srebp)-1c and Srebp-2 mRNA expression. E and F, peroxisomal proliferator activated protein receptor-α (Ppara) and PPAR-Coactivator-1α (Pgc1a) mRNA expression. G and H, ethanolamine kinase (Etnk)-1 and Etnk-2 mRNA expression. I, CTP:phosphoethanolamine cytidylyltransferase (Pcyt2) mRNA expression. Gene expression was evaluated by quantitative real-time PCR and normalized to the eukaryotic initiation factor 2a housekeeping gene. P-values represent the significance *P < 0.05 comparing postnatal LF vs. HFS diet groups and ^§P < 0.05 comparing GDM and Lean offspring receiving the same diet as calculated by ANOVA with a Bonferroni post hoc test (n = 6 by group).