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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Diagn Microbiol Infect Dis. 2015 May 27;83(1):18–20. doi: 10.1016/j.diagmicrobio.2015.05.011

Table 1.

Results of testing for limit of detection for pathogensa

Pathogen(s) Spiked stoolb
1 week		 Whatman™ FTA Elute Card with spiked stool smearb
1 week 1 month 3 months
Enterotoxigenic E. coli (LT/ST) 105/105 103/104 102/103 103/103
Enterohemorrhagic E. coli (stx1/stx2) 105/104 103/103 106/105 102/102
Enteroaggregative E. coli 105 104 105 104
Salmonella typhimurium 104 101 104 102
Salmonella enteritidis 102 104 103 103
Shigella sonnei 103 103 102 102
Campylobacter jejuni 104 105 106 Not detected
Giardia lamblia 103 100 101 101
Cryptosporidium parvum Not detected 106 Not detected Not performed
Norovirus GIb 104 103 105 104
Norovirus GIIb 102 103 105 105
a

Concentration at which pathogen was detected by multiplex PCR (mean fluorescence intensity value for detection was ≥ 300); one sample tested for each pathogen for dilutions 100-10−3; for dilutions 10−4 and 10−5 samples were tested in triplicate and mean MFI was used to determine limit of detection

b

Bacteria LoD reported as CFU/g, parasites reported as cysts/g, norovirus reported as PFU/g