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. Author manuscript; available in PMC: 2016 Sep 10.
Published in final edited form as: Neuroscience. 2015 Jun 20;303:1–15. doi: 10.1016/j.neuroscience.2015.05.081

Figure 3. Expression of immune cell marker proteins.

Figure 3

A, B, and C: Double-labeling of immune cells with F4/80 (A, red fluorescence) and CD45 (B, green fluorescence) in the middle section of a normal cochlea. All of the cells that have the irregular shape display both F4/80 and CD45 immunoreactivities (C). D and E: CD11c immunoreactivity in the macrophages beneath the basilar membrane of a normal cochlea. CD11c immunoreactivity is not detectable in the macrophages beneath the apical section of the basilar membrane (D). By contrast, the macrophages beneath the basal section display strong CD11c immunoreactivity (arrows, E). Mesothelial cells display weaker CD11c immunoreactivity. F and G: CD14 immunoreactivity in the macrophages of the basilar membrane of a normal cochlea. The CD14 immunoreactivity is not detectable in the macrophages beneath the apical section of the basilar membrane (F). Mesothelial cells display weak florescence. In contrast, the macrophages beneath the basal section of the basilar membrane display strong CD14 immunoreactivity (arrows, G). H: MHC II immunoreactivity in the macrophages of the basilar membrane. The positively stained cells (arrows) are scatted along the basilar membrane. Bars = 25 μm. All images are oriented with the top being the lateral wall and the bottom being the osseous spiral lamina.