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. Author manuscript; available in PMC: 2016 Aug 28.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jul 10;464(3):698–704. doi: 10.1016/j.bbrc.2015.06.170

Figure 2. Heparanase protein is over-expressed in the brain of hpa-transgenic mice.

Figure 2

A) ELISA. Heparanase protein was assayed by sandwich ELISA [41] in extracts of different brain areas of hpa-Tg vs. control C57BL mice (control). Each bar graph is the mean + SD of triplicate determinations. B) Immunohistochemistry. Immunostaining with anti-heparanase pAb 733 antiserum showing intense heparanase expression primarily in Purkinje cells (PC) of the cerebellum of hpa-Tg (right panel; original magnification X20) vs. weak expression in control C57BL mice (left panel; original magnification X10). ML = molecular layer; GL = granular layer; WM = white matter; Purkinje cells = PC.