Figure 2A. Kegg Diagram interconnecting MAPK signaling with CCL2 release. CCL2 release is initiated by TNFα acting on TNFR1 and contributes to leukocyte recruitment. The factors analyzed in this study are highlighted.
Figure 2B. Effect of DADS and MAPK signaling inhibitors on CCL2 release in MDA-MB-231 cells. Additive or synergistic effects of MAPK inhibitors on DADS-treated (100μM), TNFα-treated (40ng/ml) and co-treated MDA-MB-231 cells after 24 hrs. The data are presented as CCL2 release (OD 450nm) and represent the Mean ± S.E.M. n=4. Significance of differences from the control in both groups was determined by T-test. *p<0.05.