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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mucosal Immunol. 2015 Feb 11;8(5):1131–1143. doi: 10.1038/mi.2015.4

Figure 7. SMYD3 is required to mount a protective immune response against RSV-infection.

Figure 7

(A) C57BL/6 wild type mice were infected RSV and lungs were assessed for foxp3 expression at 1, 2, 4, 6, 8, and 12 (n=3) days post-infection (dpi). WT (n=4) and SMYD3−/− (n=4) were infected with RSV and assessed at 8 dpi. Percentage of Foxp3+ in gated CD3+CD4+CD25+T cells (B), percentage of activated CD69+ in gated CD3+CD4+T cells (C), and percentage of CD4+IL17+ TH17 cells in gated CD3+T cells (D). (E) Lungs from RSV-infected WT and SMYD3−/− mice were homogenized, mRNA was extracted and expression of cytokine was assessed by q-RT-PCR. (F) PAS staining of lung sections of WT (left panel) and SMYD3−/− mice (right panel) infected with RSV at 8 dpi. (G) muc5ac and gob5a mRNA in the lungs of RSV-infected WT and SMYD3−/− mice. Data show means ± SEM of triplicate wells and are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001.