Characterization of cultured hEPCs and B2R expression in hEPCs. A. At 7 d following isolation, the adherent cells intensively took up acLDL and bound an endothelial-specific lectin, as assessed by using fluorescence microscopy (original magnification: 400 ×). B. The hEPCs of passage 3 were positive for CD34, KDR, and CD105, but negative for CD45 and CD68, as determined by immunofluorescence (original magnification: 400 ×). C. Co-localization of B2R with the progenitor lineage marker CD34 in the hEPCs of passage 3 (original magnification: 400 ×). D. B2R expression in hEPCs was detected via flow cytometry.