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Effect of TUS on HUVECs tube formation. After 6 h, tube-like structures of the control group (A), TUS group (B) and TUS+L-NAME group (C) were photographed at 100 x magnification and tube length was measured as described (D). TUS significantly promoted the tube formation of HUVECs. Co-incubation with L-NAME, the specific inhibitor of eNOS, dramatically suppressed the tube formation. Values are mean ± SEM; n = 5, ** means p < 0.01. Scale bars = 200 μm.
