Effects of TUS on PI3K-Akt-eNOS signal pathway in HUVECs. Quantitative results of protein expression of eNOS, p-eNOS, p-Akt and total Akt. TUS promoted the expression of eNOS, p-eNOS, and p-Akt protein significantly. TUS-induced protein changes were significantly suppressed by LY294002 treatment. Data of Western blotting were represented as as percentages of expression in control group. Values are mean ± SEM; n = 5, * means p < 0.05, **means p < 0.01.