Figure 6.

In rodent OLG cultures, inhibition of the lysoPLD activity of ATX leads to a reduction in HDAC activity. A, Experimental design. Timing of histone deacetylation is marked as described by Marin-Husstege et al. (2002). OPC, OLG progenitor. B–D, Bar graphs showing HDAC activity as determined by using the fluorogenic substrate Boc-Lys(Ac)-AMC. Control (vehicle-treated) values were set to 100% (see horizontal line in B and control bars in C, D), and experimental values were calculated accordingly. Data represent means ± SEMs. ns, Not significant; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.