Figure 7.

In rodent OLG cultures, inhibition of the lysoPLD activity of ATX leads to an increase in nuclear histone acetylation at H3K9. A, B, Representative confocal images of differentiating OLGs treated with vehicle (control, top) or HA130 (bottom) and immunolabeled using O4 hybridoma supernatants (A, B) and anti-acetyl-histone H3K9 antibodies (A). Nuclei are visualized via staining with Hoechst (A, B). Scale bars: A, 20 μm; B, 100 μm. C, Box and whisker plot depicting nuclear acetyl-H3K9 levels as assessed by determining fluorescence intensities shown in arbitrary units (a.u.). The plot depicts medians and quartiles of three independent experiments. Whiskers represent the 10th and 90th percentiles. ***p ≤ 0.001. D, Bar graph illustrating acetyl-H3K9 levels as assessed by Western blot analysis. A representative Western blot image is shown in the inset (top right). For the bar graph, control (vehicle-treated) values were set to 100% (see control bar), and experimental values were calculated accordingly. Data represent means ± SEMs. *p ≤ 0.05.