Figure 8.

In rodent OLG cultures, LPA rescue of ATX–lysoPLD activity inhibition requires the activity of class I HDAC members HDAC1 and HDAC2 but not the class II HDAC member HDAC6. A, Experimental design. Timing of histone deacetylation is marked as described by Marin-Husstege et al. (2002). OPC, OLG progenitor. B, C, Bar graphs depicting mRNA levels for the OLG differentiation genes Ugt8 (B) and Cnp (C) as determined by real-time RT-qPCR analysis. Control (vehicle-treated) values were set to 100% (see horizontal line), and experimental values were calculated accordingly. Data represent means ± SEMs. ns, Not significant; *p ≤ 0.05, **p ≤ 0.01. TubA, Tubastatin A.