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. 2015 Aug 4;6:7806. doi: 10.1038/ncomms8806

Figure 6. Flow cytometry and cytokine secretion analysis of small intestinal IELs.

Figure 6

The total number of live (a) CD45+ cells, (b) CD45+CD3+γδTCR+ cells, (c) CD45+CD3+CD8+γδTCR+ cells and (d) CD45+CD3+NK1.1+ cells isolated from the upper 5 cm of the small intestine (duodenum) in BG-exposed and unexposed mice on each diet. Data from the unexposed malnourished and control mice are representative of three independent experiments pooled (n=13). Data from the BG-exposed malnourished and control mice are representative of two independent experiments (n=8). Bars indicate the mean values (*P<0.05, one-way analysis of variance (ANOVA) with post hoc Tukey's test). (e) Concentrations of TNF-α, IFN-γ and IL-17A after stimulation of cultured IELs for 48 h. Data are pooled from two independent experiments (n=8 per group). Bars indicate the mean values (*P<0.05, one-way ANOVA with post hoc Tukey's test). CON, control; MAL, malnourished.