Figure 1. Reduced adiposity and adipocyte maturity in mice lacking 14-3-3ζ.

(a) Weights of e19.5 wild-type (WT) and 14-3-3ζ knockout (14-3-3ζKO) (n=3 per group). (b) Body weights of 14-3-3ζKO mice and littermate control wild-type (WT) mice (n=10–12 per group). (c) DEXA body composition analysis of WT and 14-3-3ζKO at 24 weeks of age (n=5–7 per group). (d) Weights of inguinal, brown adipose (BAT), gonadal and peri-renal fat pads relative to total body weight of male WT and 14-3-3ζKO mice (n=4 per genotype). (e,f) Analysis of white adipocyte area (e) and size distribution (f) of inguinal fat tissue, as assessed by perilipin staining, from 26 week old WT and KO mice (n=3 per genotype). (g–i) Immunoblot (g) and quantitative PCR measurements (h,i) of pro-adipogenic factors or mature adipocyte markers, respectively, from gonadal fat pads (n=4–6 per group). (j) Comparison of remaining 14-3-3 isoforms in gonadal white adipose tissue from WT and 14-3-3ζKO mice (n=5 per group). (k–n) Intraperitoneal glucose (k,m; 2 g kg⁻¹ b.w.) and insulin (l,n; 1.5 U kg⁻¹) tolerance tests on WT and 14-3-3ζKO littermates at 10 (k,l) and 25 (m,n) weeks of age. Area-under-the-curve measurements are shown (n=7–8 mice per group). Error bars represent s.e.m. Significant differences between WT and KO mice are indicated by *P<0.05 (assessed by Student's t-test).