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. 2015 Jul 29;6:7671. doi: 10.1038/ncomms8671

Figure 3. siRNA-mediated knockdown of all 14-3-3 proteins reveals the 14-3-3ζ as the critical regulator of adipocyte differentiation.

Figure 3

(a) 3T3-L1 pre-adipocytes were pretreated with a cell-permeable pan-14-3-3 inhibitor (14-3-3i) and differentiated by MDI. Differentiation was visualized by Oil-Red-O incorporation (n=4 independent experiments, *P<0.05, assessed by one-way ANOVA). (b) 3T3-L1 pre-adipocytes were transfected with 14-3-3 isoform-specific siRNA followed by differentiation with MDI. Differentiation was measured by Oil Red-O incorporation (n=4 independent experiments, *P<0.05, assessed by Student's t-test). (c,d) Pro-adipogenic factors (c) or mature adipocyte markers (d) were measured 48 h or 7 days, respectively, after differentiation in control- (siCon) and si14-3-3ζ-transfected 3T3-L1 cells (n=4 per group, *P<0.05, assessed by one- or two-way ANOVA). Error bars represent s.e.m.