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. 2015 Jul 29;6:7671. doi: 10.1038/ncomms8671

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(a–c) Frozen sagittal sections of e18.5 embryos were stained for (a) Pref-1 alone to examine differences in the number of adipose precursor cells, (b) Pref-1 and Gli3, and (c) Pref-1 and p27^Kip1. LipidTOX Deep-red was used to label mature adipocytes within the embryo (representative images of n=3 per genotype; Scale bar=10μm (a) or 50 μm (b,c)).