Figure 2. Quantitative PCR and ChIP analyses of plants vernalized at 5°C, 8°C and 14°C.
Changes in FLC (A), VIN3 (B) and (C) FT expression were determined directly after 6 weeks of cold exposure (T0) and again after 10 (T10) and 30 (T30) days subsequent growth at 20°C. Two-tailed Student's t-test results: *p < 0.05, ***p < 0.005. n = 3. Error bars = ±S.D. (D) H3K27me3 levels over the FLC locus were higher for Lov-1 after 6 weeks vernalization at 8°C than 14°C or 5°C (samples were harvested 30 days post cold). **** p < 0.0001, Wilcoxon matched-pairs signed rank test on measurements for 12 primer pairs. Error bars = ±S.E.M. NV = nonvernalized, DNF = did not flower and ns = not significant.
DOI: http://dx.doi.org/10.7554/eLife.06620.006
Figure 2—source data 1. Primers used for qPCR ChIP.
elife06620s001.pdf (114.7KB, pdf)
DOI: 10.7554/eLife.06620.007
Figure 2—figure supplement 1. FLC expression determined after 4 weeks of vernalization at a range of temperatures.
Quantitative PCR (qRT-PCR) analysis showing FLC expression levels before cold (hatched), after 4 weeks vernalization (white) and after 10 days (grey) and 30 days (black) subsequent growth at 20°C. *p = 0.0038 two-tailed Student's t-test. n = 3. NV = non-vernalized.