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Melanin production and laccase activity. (A) Growth of C. neoformans strains on L-3,4 dihydroxyphenylalanine (L-DOPA) agar plates at 30°C. (B) Laccase activity of C. neoformans strains. A p-value <0.05 is indicated with an asterisk. (C) LAC1 gene expression. Cells from WT, cln1 and CLN::CLN1 strains were grown in minimal media for 3 days at 30°C and RNA samples were isolated. cDNAs were obtained and relative expression of the LAC1 gene was measured by real time PCR.
