FIGURE 7.

Effect of rapamycin on melanization and growth of C. neoformans. (A) Panels show cultures of C. neoformans in L-DOPA liquid medium containing different rapamycin concentrations at 0 and 48 h. Cultures with DMSO were carried out in parallel since rapamycin was dissolved in DMSO. (B) Growth of C. neoformans in L-DOPA medium containing different rapamycin concentrations. Optical density (OD) was measured at 540 nm every hour for 48 h. Black line, growth in control medium (minimal medium + DMSO); red line, rapamycin 0.5 μg/mL; green line, rapamycin 1 μg/mL.