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. 2015 Aug 1;12(8):655–667. doi: 10.7150/ijms.12460

Figure 3.

Figure 3

Inhibition of autophagy by 3-MA dramatically attenuated LPS-induced AKI in mice. The mice were pretreated i.p. without or with 3-MA (15 mg/kg in 0.1 mL of 0.9 % normal saline)(n=8) for 1h, followed by exposure to LPS (10 mg/kg in 0.1 mL of 0.9 % normal saline)(n=8) for 24h. The homogenates of one side of kidney were used to examined the expression levels of Beclin-1(A) and LC3-II (B). The other side of kidney was used for immunohistochemical staining for LC3-II to examine its expression in renal cortex (C). A. Left panel, representative Western blots for Beclin-1; Right panel, quantification of Beclin-1 protein by densitometry (n=3); B. Left panel, the representative Western blots for LC3-II; Right panel, quantification of LC3-II protein by densitometry (n=3); C. left panel, immunohistochemical staining for LC3-II protein in renal cortex; right panel, quantitative analysis of the optical intensity of the immunohistochemical staining image of LC3-II protein. *P<0.05 vs. CON; # P<0.05 vs. LPS.