Fig 1.

Induction of HO-1 in human osteosarcoma cells following treatment with ATO. Cells were exposed to 10 μM ATO for 24 hours. The mRNA and protein were then analyzed by RT-QPCR and western blot, respectively. The amount of mRNA and protein were normalized to β-actin mRNA or protein. Relative fold activation was calculated based on the ratio of the normalized values of the cells treated with ATO to that of the control cells. The data are expressed as the mean ± SD, N=6, *P<0.05 and **P<0.01 versus control cells without exposure to ATO.