Fig 2.

Actinomycin-D abolished HO-1 induction in MG63 cells following their treatment with ATO. Cells were exposed to ATO (10 μM) for 24 hours in the presence of Act-D or vehicle. Act-D (10 μg/ml) was added to the cells 30 min earlier than addition of ATO and TSA (500ng/ml) was added to the cells at the same time as the addition of ATO. HO-1 mRNA was analyzed by RT-QPCR and the amount of mRNA was normalized to β-actin mRNA. Relative fold activation was obtained based on the ratio of the normalized values of the cells treated with ATO to that of the untreated control cells. The data are expressed as the mean ± SD, N=6, **P<0.01 versus control cells without exposure to ATO.