Fig 3.

The exposure to ATO caused nuclear translocation of NFE2L2 in MG63 cells. Cells were exposed to ATO for 24 hours at the concentration indicated in the figure. Total cellular and nuclear proteins were separated by PAGE and subjected to Western blot analysis. For photography and densitometric analysis, Quantity One software (Version 4.6.3, Bio Rad, USA) was used. NFE2L2 proteins in total cellular and nuclear proteins were normalized for loading to β-actin and Histone H3 protein, respectively and expressed relative to the value of the untreated control cells. Fig. 3A and Fig. 3B are representative photographs of Western blots of total cellular and nuclear protein, respectively. Fig. 3C and Fig. 3D are statistical analysis of quantified western blots. The data are expressed as the mean ± SD, N=6, **P<0.01 versus the control cells without exposure to ATO.