Fig 3.

Decrease of colorectal cancer cell viability via knocking-down Nampt. (A) HT29, Caco2, and HCT116 cells were transfected with siRNA for 48 h and Nampt protein was detected by Western blotting. (B) Densitometry of signals in Western blot. (C) Cell viability (%) was detected by CCK-8 assay at 48 h post-transfection. (D) Cell viability (%) was detected by CCK-8 assay at 72 h post-transfection. C control cells without transfection, Lipo control cells treated with transfection reagent Lipofectamine RNAiMAX alone, NC cells transfected with negative control of oligonucleotides, siR cells transfected with Nampt-siRNA. *, P < 0.01 (siR vs. NC).