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. 2015 Aug 12;5:13013. doi: 10.1038/srep13013

Figure 4. Rhus coriaria alters the expression of cell cycle regulators and inhibits proliferative recovery of breast cancer cells.

Figure 4

(A) Alteration in proteins associated with cell cycle regulation and senescence in MDA-MB-231 cells, in response to Rhus coriaria. Cells were treated with or without the indicated concentrations of RCE for 48 h, and the protein level of Cyclin D1, PCNA, pRB, c-myc, p16, p21 and p27 was examined by western blotting. (B) Time-course accumulation of p21 in treated MDA-MB-231 cells. Cells were treated with 400 μg/mL RCE and a protein level of p21 was determined by western blot at different time-point (6, 12, 24 and 48 h) post-treatment. (C) Inhibition of cell viability recovery after RCE removal. MDA-MB-231 cells were exposed to RCE for 24 h, then, cells were washed to remove RCE and allowed to grow for another 48 h in fresh complete media. Cell viability was monitored using the Muse Cell Analyzer as described in material and methods. Data represent the mean ± SEM of three independent experiments.